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cat drg00  (R&D Systems)


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    R&D Systems cat drg00
    Cat Drg00, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+rage+quantikine+elisa+kit/Human+RAGE+Quantikine+ELISA+Kit/pm41754134-89-11-9
    Average 95 stars, based on 119 article reviews
    cat drg00 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Determination of the effects of advanced glycation end products receptor polymorphisms and its activation on structural cell responses and inflammation in asthma.
    Article Snippet: .. Amounts of RAGE protein in plasma were determined according to the instructions of manufacturers in randomly selected asthmatic and control samples using a commercially available Human RAGE Quantikine ELISA Kit (Quantikine, R&D, Minneapolis, USA) [10]. .. BEAS-2B (ATCC CRL-9609), an immortalized human lung epithelial cell line, CCD-16Lu (ATCC CCL-204) human lung fibroblast cell line and HULEC5a (ATCC CRL-3244) microvascular endothelial cell that was isolated from the lungs were used as a sources of lung structural cells.

    Article Title: Alterations of senescence-associated markers in patients with non-syndromic cleft lip and palate
    Article Snippet: .. The plasma p16 was determined using the Human p16 ELISA kit (Cat# MBS015625, MyBioSource, USA), the plasma AGEs level was determined using the Human AGEs ELISA kit (Cat# MBS267540, Cusabio, USA), and the plasma RAGEs level was detected using the Human RAGE Quantikine ELISA kit (Cat# DRG00, R&D Systems, USA). ..

    Article Title: “Soluble form of the receptor for advanced glycation end products (sRAGE) as a marker of inflammation in pediatric cystic fibrosis population, a pilot study.”
    Article Snippet: .. Plasma sRAGE level were measured by Human RAGE Quantikine ELISA Kit (R&D Systems Inc., Minneapolis, MN), using an enzyme-linked polyclonal antibody specific for human RAGE (extracellular domain). ..

    Control:

    Article Title: Determination of the effects of advanced glycation end products receptor polymorphisms and its activation on structural cell responses and inflammation in asthma.
    Article Snippet: .. Amounts of RAGE protein in plasma were determined according to the instructions of manufacturers in randomly selected asthmatic and control samples using a commercially available Human RAGE Quantikine ELISA Kit (Quantikine, R&D, Minneapolis, USA) [10]. .. BEAS-2B (ATCC CRL-9609), an immortalized human lung epithelial cell line, CCD-16Lu (ATCC CCL-204) human lung fibroblast cell line and HULEC5a (ATCC CRL-3244) microvascular endothelial cell that was isolated from the lungs were used as a sources of lung structural cells.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Determination of the effects of advanced glycation end products receptor polymorphisms and its activation on structural cell responses and inflammation in asthma.
    Article Snippet: .. Amounts of RAGE protein in plasma were determined according to the instructions of manufacturers in randomly selected asthmatic and control samples using a commercially available Human RAGE Quantikine ELISA Kit (Quantikine, R&D, Minneapolis, USA) [10]. .. BEAS-2B (ATCC CRL-9609), an immortalized human lung epithelial cell line, CCD-16Lu (ATCC CCL-204) human lung fibroblast cell line and HULEC5a (ATCC CRL-3244) microvascular endothelial cell that was isolated from the lungs were used as a sources of lung structural cells.

    Article Title: Assessment and prediction models for the quantitative and qualitative reserve of the ovary using machine learning
    Article Snippet: .. Serum concentrations of advanced glycation-end products (AGEs), soluble receptor of AGEs (sRAGE), growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) were measured using an OxiSelect AGE Competitive ELISA Kit (Cell BioLabs, Inc., San Diego, CA, USA), human RAGE Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), Human GDF9 ELISA Kit (LifeSpan BioSciences, Inc., Lynnwood, WA, USA), and Human BMP15 ELISA Kit (CUSABIO, Houston, TX, USA), respectively. .. Oxidative stress and antioxidant potential were measured using a Diacron reactive oxygen metabolites (d-ROMs)/biological antioxidant potential (BAP) Kit and Free Radical Elective Evaluator (FREE Carrio Duo; Diacron International srl, Grosseto, Italy).

    Article Title: Low Serum sRAGE and MASLD Are Associated and Share a Similar Gut Microbiota Profile: A Prospective and Cross-Sectional Analysis.
    Article Snippet: .. Serum sRAGE levels (all isoforms) were measured at both time points using a human RAGE Quantikine ELISA kit (R&D Systems, MN) in frozen serum stored at −80°C. ..

    Article Title: Symptoms of COPD in the absence of airflow obstruction are more indicative of pre-COPD than overdiagnosis
    Article Snippet: For biomarkers measured using ELISA, GraphPad Prism 8 version 9.0 (Boston, MS, USA) was used to create standard curves and interpolation of unknown concentrations. .. Symptoms of COPD in the absence of airflow obstruction are more indicative of pre-COPD than overdiagnosis – supplementary material Description of commercially-available kits used for biomarker quantification CC-16 was measured using Human Uteroglobin Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA); RAGE using Human RAGE Quantikine ELISA kit (R&D Systems); SP-D using Luminex® Discovery Assay (LXSAHM-01) (Bio-Techne Limited, Minneapolis, MN, USA); MMP-8, MMP9 and MMP-12 using Luminex® Human MMP Premixed Kit (Bio-Techne Limited); PAI-1 using Luminex® Human Obesity Premixed Kit (Bio-Techne Limited); CCL18 using Invitrogen PARC/CCL18 Human ELISA kit (Fisher Scientific, Waltham, MA, USA). .. For biomarkers measured using ELISA, GraphPad Prism 8 version 9.0 (Boston, MS, USA) was used to create standard curves and interpolation of unknown concentrations.

    Article Title: Alterations of senescence-associated markers in patients with non-syndromic cleft lip and palate
    Article Snippet: .. The plasma p16 was determined using the Human p16 ELISA kit (Cat# MBS015625, MyBioSource, USA), the plasma AGEs level was determined using the Human AGEs ELISA kit (Cat# MBS267540, Cusabio, USA), and the plasma RAGEs level was detected using the Human RAGE Quantikine ELISA kit (Cat# DRG00, R&D Systems, USA). ..

    Article Title: Symptoms of COPD in the absence of airflow obstruction are more indicative of pre-COPD than overdiagnosis
    Article Snippet: .. CC-16 was measured using Human Uteroglobin Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA); RAGE using Human RAGE Quantikine ELISA kit (R&D Systems); SP-D using Luminex® Discovery Assay (LXSAHM-01) (Bio-Techne Limited, Minneapolis, MN, USA); MMP-8, MMP9 and MMP-12 using Luminex® Human MMP Premixed Kit (Bio-Techne Limited); PAI-1 using Luminex® Human Obesity Premixed Kit (Bio-Techne Limited); CCL18 using Invitrogen PARC/CCL18 Human ELISA kit (Fisher Scientific, Waltham, MA, USA). .. For biomarkers measured using ELISA, GraphPad Prism 8 version 9.0 (Boston, MS, USA) was used to create standard curves and interpolation of unknown concentrations.

    Article Title: “Soluble form of the receptor for advanced glycation end products (sRAGE) as a marker of inflammation in pediatric cystic fibrosis population, a pilot study.”
    Article Snippet: .. Plasma sRAGE level were measured by Human RAGE Quantikine ELISA Kit (R&D Systems Inc., Minneapolis, MN), using an enzyme-linked polyclonal antibody specific for human RAGE (extracellular domain). ..

    Article Title: Advanced glycation end products and their soluble receptor (sRAGE) in patients with Hashimoto’s thyroiditis on levothyroxine substitution
    Article Snippet: .. The serum AGEs levels were determined by autofluorescence on a multi-mode microplate reader (Biotek Synergy H1, Agilent Technologies, Santa Clara, CA, USA), and the sRAGE levels by ELISA method (Human RAGE Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. The serum free thyroxine (fT4) and free triiodothyronine (fT3) and thyroid stimulating hormone (TSH) levels were measured using electrochemiluminescence immunoassays (FT4 G2 Elecsys, FT3 Elecsys, TSH Elecsys, Roche Diagnostics GmbH, Mannheim, Germany).

    Competitive ELISA:

    Article Title: Assessment and prediction models for the quantitative and qualitative reserve of the ovary using machine learning
    Article Snippet: .. Serum concentrations of advanced glycation-end products (AGEs), soluble receptor of AGEs (sRAGE), growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) were measured using an OxiSelect AGE Competitive ELISA Kit (Cell BioLabs, Inc., San Diego, CA, USA), human RAGE Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), Human GDF9 ELISA Kit (LifeSpan BioSciences, Inc., Lynnwood, WA, USA), and Human BMP15 ELISA Kit (CUSABIO, Houston, TX, USA), respectively. .. Oxidative stress and antioxidant potential were measured using a Diacron reactive oxygen metabolites (d-ROMs)/biological antioxidant potential (BAP) Kit and Free Radical Elective Evaluator (FREE Carrio Duo; Diacron International srl, Grosseto, Italy).

    Biomarker Discovery:

    Article Title: Symptoms of COPD in the absence of airflow obstruction are more indicative of pre-COPD than overdiagnosis
    Article Snippet: For biomarkers measured using ELISA, GraphPad Prism 8 version 9.0 (Boston, MS, USA) was used to create standard curves and interpolation of unknown concentrations. .. Symptoms of COPD in the absence of airflow obstruction are more indicative of pre-COPD than overdiagnosis – supplementary material Description of commercially-available kits used for biomarker quantification CC-16 was measured using Human Uteroglobin Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA); RAGE using Human RAGE Quantikine ELISA kit (R&D Systems); SP-D using Luminex® Discovery Assay (LXSAHM-01) (Bio-Techne Limited, Minneapolis, MN, USA); MMP-8, MMP9 and MMP-12 using Luminex® Human MMP Premixed Kit (Bio-Techne Limited); PAI-1 using Luminex® Human Obesity Premixed Kit (Bio-Techne Limited); CCL18 using Invitrogen PARC/CCL18 Human ELISA kit (Fisher Scientific, Waltham, MA, USA). .. For biomarkers measured using ELISA, GraphPad Prism 8 version 9.0 (Boston, MS, USA) was used to create standard curves and interpolation of unknown concentrations.



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    Plasma concentrations of AGEs ( A ), <t>sRAGE</t> ( B ), and AGE/sRAGE ratio ( C ) in patients with type 2 diabetes with neuropathy before and after six months of 600 mg/day alphalipoic acid (ALA) treatment and in diabetic controls without neuropathy. Solid lines represent medians. Magenta dots represent data from neuropathic patients before ALA treatment, green dots represent data from neuropathic patients after six months of ALA treatment, and grey dots represent data of age-, gender-, and BMI-matched diabetic controls. p -values were calculated by the Wilcoxon matched paired test in neuropathic patients before and after ALA, and by the Mann–Whitney U -test between neuropath patients and diabetic controls. Abbreviations: ALA, alphalipoic acid; AGE, advanced glycation end product; AU, autofluorescence; sRAGE, soluble receptor of advanced glycation end products.
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    Image Search Results


    Basal solubilized receptor for advanced glycation end products (sRAGE; A), solubilized Toll-like receptor 4 (sTLR4, C) pre and post 12-week intervention in the control (CON) and aerobic exercise training (AE) groups. Respective absolute changes in basal sRAGE (B) and sTLR4 (C) and percentage changes in sRAGE (E) and sTLR4 (E) were calculated. *P < 0.05 vs Pre, # P < 0.05 vs CON. Statistical analyses performed include mixed model regression analyses with Bonferroni post hoc (panels A & C) and two-tailed t-test (panels B, D, & E).

    Journal: medRxiv

    Article Title: Aerobic Exercise Training Elevates Circulating sRAGE via Modulation of Sheddase Regulation in Adults with Type 2 Diabetes

    doi: 10.1101/2025.09.10.25335517

    Figure Lengend Snippet: Basal solubilized receptor for advanced glycation end products (sRAGE; A), solubilized Toll-like receptor 4 (sTLR4, C) pre and post 12-week intervention in the control (CON) and aerobic exercise training (AE) groups. Respective absolute changes in basal sRAGE (B) and sTLR4 (C) and percentage changes in sRAGE (E) and sTLR4 (E) were calculated. *P < 0.05 vs Pre, # P < 0.05 vs CON. Statistical analyses performed include mixed model regression analyses with Bonferroni post hoc (panels A & C) and two-tailed t-test (panels B, D, & E).

    Article Snippet: sRAGE was quantified via sRAGE ELISA (DRG000, R&D Systems, Minneapolis, MN) according to manufacturer’s instructions.

    Techniques: Control, Two Tailed Test

    Plasma concentrations of AGEs ( A ), sRAGE ( B ), and AGE/sRAGE ratio ( C ) in patients with type 2 diabetes with neuropathy before and after six months of 600 mg/day alphalipoic acid (ALA) treatment and in diabetic controls without neuropathy. Solid lines represent medians. Magenta dots represent data from neuropathic patients before ALA treatment, green dots represent data from neuropathic patients after six months of ALA treatment, and grey dots represent data of age-, gender-, and BMI-matched diabetic controls. p -values were calculated by the Wilcoxon matched paired test in neuropathic patients before and after ALA, and by the Mann–Whitney U -test between neuropath patients and diabetic controls. Abbreviations: ALA, alphalipoic acid; AGE, advanced glycation end product; AU, autofluorescence; sRAGE, soluble receptor of advanced glycation end products.

    Journal: Biomedicines

    Article Title: Alpha-Lipoic Acid Treatment Reduces the Levels of Advanced End Glycation Products in Type 2 Diabetes Patients with Neuropathy

    doi: 10.3390/biomedicines13020438

    Figure Lengend Snippet: Plasma concentrations of AGEs ( A ), sRAGE ( B ), and AGE/sRAGE ratio ( C ) in patients with type 2 diabetes with neuropathy before and after six months of 600 mg/day alphalipoic acid (ALA) treatment and in diabetic controls without neuropathy. Solid lines represent medians. Magenta dots represent data from neuropathic patients before ALA treatment, green dots represent data from neuropathic patients after six months of ALA treatment, and grey dots represent data of age-, gender-, and BMI-matched diabetic controls. p -values were calculated by the Wilcoxon matched paired test in neuropathic patients before and after ALA, and by the Mann–Whitney U -test between neuropath patients and diabetic controls. Abbreviations: ALA, alphalipoic acid; AGE, advanced glycation end product; AU, autofluorescence; sRAGE, soluble receptor of advanced glycation end products.

    Article Snippet: Day-to-day variation (5 days) was 10.5% (n = 35). sRAGE levels were measured by the ELISA method (Human sRAGE Quantikine ELISA kit, R&D Systems Europe Ltd., Abington, UK) according to the manufacturer’s instruction.

    Techniques: Clinical Proteomics, MANN-WHITNEY

    Correlations of AGE and  sRAGE  with vascular markers in neuropathic patients before and after ALA treatment.

    Journal: Biomedicines

    Article Title: Alpha-Lipoic Acid Treatment Reduces the Levels of Advanced End Glycation Products in Type 2 Diabetes Patients with Neuropathy

    doi: 10.3390/biomedicines13020438

    Figure Lengend Snippet: Correlations of AGE and sRAGE with vascular markers in neuropathic patients before and after ALA treatment.

    Article Snippet: Day-to-day variation (5 days) was 10.5% (n = 35). sRAGE levels were measured by the ELISA method (Human sRAGE Quantikine ELISA kit, R&D Systems Europe Ltd., Abington, UK) according to the manufacturer’s instruction.

    Techniques: